1．基本信息
沈清武，男，1973年10生，湖南省沅陵县人，教授，博士生导师。1997年本科毕业于南京农业大学。1997年6月至2001年8月于湖南省安江农业学校（现湖南省怀化职业技术学院）任教。2001年9月进入中国农业大学学习，2004年6月获硕士学位。同年进入美国怀俄名大学（University of Wyoming）动物科学系攻读博士学位，2007年5月获得哲学博士学位。2007年5月至2009年8月于普渡大学（Purdue University）从事博士后研究工作，研究方向为肌肉生物学。2009年9月至2010年8月于华盛顿大学(University of Washington, Seattle)医学院从事博士后研究工作，研究方向为肌肉干细胞生物学。
2．研究方向
本人的主要研究方向为肌肉干细胞生物学，动物肌肉生长发育的分子调控机理。主要包括以下三个方面：
（1）动物肌肉生长发育，特别胎儿阶段肌肉生长发育的分子机理及调控。大量研究表明，
哺乳动物（包括人类）肌肉中肌肉纤维的数量在动物出生前就已经决定。动物出生后肌纤维的数量不再增加，肌肉的生长取决于肌纤维大小的增长。也就是说，产肉动物肉品的产量和质量在胎儿出生前就已经部分决定。因此，对胎儿阶段肌肉生长发育分子机理的研究对提高产肉动物的产量和质量具有重要的理论指导意义。我们以前的研究表明母羊营养缺乏和营养过剩都降低了胎儿肌细胞的数量和肌肉量，增加了后代二型糖尿病发生的可能性。虽然我们也发现母体营养缺乏提高了胎儿肌肉中pTEN 的表达，营养过剩降低了胎儿肌肉中腺嘌呤单核苷酸激活的激酶（AMP-activated protein kinase, AMPK）的表达和活性，但其分子调控机理还不清楚。因此，AMPK和Wnt信号通道对肌肉生长发育的调控及其分子机理将是本人近期主要研究的方向之一。
（2）肌纤维类型的决定机理其调控。成年动物骨骼肌中主要有I, IIA, IIB和IID(X)四种
类型的肌肉纤维。不同的纤维类型具有不同的能量代谢方式和收缩特点。因此肌肉中肌纤维类型的组成不仅影响动物屠宰后的肉品品质，也影响动物的运动能力。因此明了肌纤维类型的决定机理，在动物生产中能为控制肌纤维类型组成，改善肉品品质提供理论基础。在竞技运动中，能指导运动员训练，调控肌纤维类型组成，提高运动能力。
（3）肌肉中脂肪细胞的来源及脂肪形成机理的研究。
3．主要学术论文
专业期刊
· Shen, Q. W., & D. R. Swartz. 2010. Influence of salt and pyrophosphate on bovine fast and slow myosin S1 dissociation from actin. Meat Science. 84(3): 364-370.
·Yang, Z., M. Yamazaki, Q. W. Shen, and D. R. Swartz (2008). Difference between cardiac and skeletal troponin interaction with the thin filament probed by troponin exchange in skeletal myofibrils. Biophysical Journal, in press. (IF: 4.757; Cited: N/A)
·Forhead, A.J., C. A. Lamb, K. L. Franko, D. M. O\'Connor, F. B. P. Wooding, R. L. Cripps, S. Ozanne, D. Blache, Q. W. Shen, M. Du, and A. L. Fowden.(2008). Role of leptin in the regulation of growth and carbohydrate metabolism in the ovine fetus during late gestation. Journal of Physiology, 586(9):2393-403. (IF: 6.268; Cited: 0)
·Li, pinglan, Qingwu Shen, Ziyu Liu, Peng Fu, Wei Zhou. 2008. A newly isolated strain Pediococcus parvulus from Xuanwei ham, a traditional Chinese fermented meat product. International Journal of Food Science & Technology. 43(8): 1387-1394. (IF: 0.832; Cited: 0)
· Shen, Q. W., Jun Ren, and M. Du. 2007. Ca2+/calmodulin-dependent protein kinase kinase is involved in AMP-activated protein kinase activation by a-lipoic acid in C2C12 myotubes. American Journal of Physiology-Cell Physiology. 293(4): 1395-1403. (IF: 4.334; Cited: 5)
· Keith R. Underwood, Junfeng Tong, Mei J. Zhu, Qingwu W. Shen, Warrie J. Means, Stephen P. Ford, Steven I. Paisley, Bret W. Hess, and Min Du. 2007. Relationship between kinase phosphorylation, muscle fiber typing and glycogen accumulation in Longissimus dorsi muscle of beef cattle with high and low intramuscular fat. Journal of Agricultural and Food Chemistry. 55(23):9698-9703. (IF: 2.322; Cited: 2)
· Shen, Q. W., D. Gerrard and M. Du. 2007. Compound C, an inhibitor of AMP-activated protein kinase, inhibits glycolysis in mouse longissimus dorsi postmortem. Meat Science. 78: 323-330. (IF: 1.84; Cited: 1)
·Shen, Q. W., K. R. Underwood, W. J. Means, R. J. McCormick, and M. Du. 2007. Halothane gene, energy metabolism, AMP-activated protein kinase, and glycolysis in postmortem pig longissimus dorsi muscle. Journal of Animal Science. 85:1054-1061. (IF: 1.983; Cited: 3)
·Min Du, Q. W. Shen, Mei J. Zhu, and Stephen P. Ford. 2007.Leucine stimulates mTOR signalling in C2C12 myoblast cells through inhibition of AMP-activated protein kinase. Journal of Animal Science. 85(4):919-927. (IF: 1.983; Cited: 4)
·Shen, Q. W., M. Du, and J. Ren. 2006. Fitness or fatness: the debate continues for AMP-activated protein kinase in heart function. Current Cardiology Reviews. 2: 117-123. (IF: N/A; Cited: N/A)
·Shen, Q. W., W. J. Means, K. Underwood, S. Thompson, Mei J. Zhu, R. J. McCormick, S.P. Ford, M. Ellis, and M. Du. 2006. Early post-mortem amp-activated protein kinase (ampk) activation leads to phosphofructokinase-2 and-1 (pfk-2 and pfk-1) phosphorylation and the development of pale, soft, and exudative (pse) conditions in porcine longissimus muscle. Journal of Agricultural and Food Chemistry 54: 5583-5589. (IF: 2.322; Cited: 9)
·Shen, Q. W., W. J. Means, S. A. Thompson, K. R. Underwood, R. J. McCormick, S. P. Ford, and M. Du. 2006. Pre-slaughter transport, amp-activated protein kinase, glycolysis, and quality of pork loin. Meat Science 74: 388-395. (IF: 1.84; Cited: 5)
· Shen, Q. W. , C. S. Jones, N. Kalchayanand, M. J. Zhu, and M. Du. 2005. Effect of dietary alpha-lipoic acid on growth, body composition, muscle ph, and amp-activated protein kinase phosphorylation in mice. Journal of Animal Science 83: 2611-2617. (IF: 1.983; Cited: 9)
· Shen, Q. W.,and M. Du. 2005. Effects of dietary alpha-lipoic acid on glycolysis of postmortem muscle. Meat Science 71: 306-311. (IF: 1.84; Cited: 7)
· Shen, Q. W., and M. Du. 2005. Role of amp-activated protein kinase in the glycolysis of postmortem muscle. Journal of the Science of Food and Agriculture 85: 2401-2406. (IF: 1.026; Cited: 8)
· Du, M., Q. W. Shen, and M. J. Zhu. 2005. Role of beta-adrenoceptor signaling and amp-activated protein kinase in glycolysis of postmortem skeletal muscle. Journal of Agricultural and Food Chemistry 53: 3235-3239. (IF: 2.322; Cited: 8)
书本章节
· Shen, Qingwu W., Min Du, and Warrie J. Means. 2009. Regulation of postmortem glycolysis and meat quality. In Applied Muscle Biology and Meat Science. Boca Raton, FL: CRC Press. In press.
· Min Du, Qingwu W. Shen, Keith R. Underwood, Jun F. Tong, and Mei J. Zhu. 2009. AMP-activated protein kinase in muscle growth, fat deposition and meat quality. In Applied Muscle Biology and Meat Science. Boca Raton, FL: CRC Press. In press.
会议摘要
· Qingwu W. Shen, Irina Kirillova, Lindsey Muir, Jeffrey Chamberlain, Andrew Shearer, Zipora Yablonka-Reuveni. 2010. Regenerative superiority of satellite cells from extraocular muscles. Skeletal muscle satellite & stem cells. Carefree, Arizona. July 17-23.
· Qingwu W. Shenand Darl R. Swartz. 2009. Ionic Strength Influence on Toponin dissociation from skeletal myofibrils. The Joint Biophysical society 53nd Annual Meeting . Boston.MA. April 28-May 4.
· Zhenyun Yang, Marie Yamazaki, Qingwu W. Shen, Yanfang Pan, and Darl R. Swartz. 2008. Differences in apparent troponin dissociation rates between cardiac and skeletal troponin in skeletal myofibrils relative to the 3-state model the thin filament regulation. The Joint Biophysical society 52nd Annual Meeting and 16th International biophysics Congress. Long Beach. CA. February 2-6.
·Bing Han, Changwei Ma, M. J. Zhu, Qingwu W. Shen, and M. Du. 2007. Leucine supplementation mitigates atrophy of non-weight-bearing skeletal muscle in rats. Experimental Biology Annual Meeting, Washington DC, April 28-May 2.
· Shen, Q. W., Jun Ren, and M. Du. 2007. Ca2+/calmodulin-dependent protein kinase kinases are mainly responsible for AMP-activated protein kinase activation by a-lipoic acid in C2C12 myotubes. Experimental Biology Annual Meeting, Washington DC, April 28-May 2.
· Shen, Q.W., Warrie J. Means, Mei J. Zhu, Keith R. Underwood, Shane A. Thompson, Richard J. McCormick, Stephen P. Ford, Michael Ellis, and Min Du. 2006 Early AMP-activated protein kinase (AMPK) activation leads to PSE meat in pork. 59th Reciprocal Meat Conference, Urbana-Champaign, June 18-21.
· Du, M., Qingwu W. Shen, and Meijun Zhu. 2006. Leucine stimulates mTOR signaling in C2C12 myoblast cells through inhibition of AMP-activated protein kinase. Experimental Biology, San Francisco, CA..
· Zhu, Mei J., Qingwu W. Shen, Stephen P. Ford, Peter W. Nathanielsz, and Min Du. 2005. Maternal nutrient restriction alters carnitine palmitoyltransferase-1 activity and myosin isoform composition of offspring skeletal muscle. Society for Gynecologic Investigation, 2006 Annual Meeting, Toronto, Canada.
· Min Du, Qingwu Shen, N. Schlabritz-Loutsevitch, Stephen P. Ford, Peter W. Nathanielsz. 2005. Global 30% nutrient restriction (NR) from 30 to 165 days gestation decrease Erk and phospho-Erk but not Akt or P-Akt in the baboon placenta. Society for Gynecologic Investigation, 2006 Annual Meeting, Toronto, Canada.
· Du, M., Qingwu W. Shen, Hyungchul Han, Mei J. Zhu, Michelle M. Schwope, Warrie J. Means, Peter W. Nathanielsz, and Stephen P. Ford. 2005. Differential signaling of growth pathways in fetal liver and muscle in response to maternal nutrient restriction. The 3rd International Congress on Developmental Origins of Health and Disease, Toronto, Canada.
· Shen, Q. W., M.J. Zhu, M. Du. 2005. Role of b-adrenoceptor signaling and AMP-activated protein kinase in the glycolysis of postmortem skeletal muscle. American Society of Animal Scientists Annual Meeting, Cincinnati, Ohio.
· Shen, Q. W., and Min Du. 2005. AMP-activated protein kinase and b-adrenoceptor in glycolysis of postmortem muscle.Department of Animal Science, 2005 Annual Report.
· Mei J. Zhu, Qingwu W. Shen, and Min Du. 2005. Protein expression profiling of skeletal muscle through 2-dimensional gel electrophoresis and protein identification by mass spectrometry. Proceeding of 51st International Congress of Meat Science & Technology, Baltimore, Maryland.
· Shen, Q. W., C. S. Jones, N. Kalchayanand, M. Du. 2004. Effect of dietary a-lipoic Acid on the growth rate and fat accumulation of mice and the ultimate pH of postmortem muscle. Colorado Nutrition Roundtable. September 23. Fort Collins. CO. USA.
4．联系方式
通讯地址：陕西杨凌西农路22号西北农林科技大学动物科技学院
邮编：712100
Email: shenqingwu@yahoo.com