姓名：陈则
性别：男
专业：医学
职称：教授、研究员
目前共带研究生人数：7人
学习简历
1992.3～1997.3日本东京大学医学博士
1982.9～1988.7上海第二医科大学医学学士
科研方面工作经历
陈则研究员1997年毕业于日本东京大学，获博士学位。曾任日本国立感染症研究所协力研究员兼日本科技厅特别研究员，2000年回国后先后被聘为湖南师范大学特聘教授,中国科学院武汉病毒研究所“百人计划”研究员。自2005年至今任上海生物制品研究所研究员。目前是中国微生物学会医学微生物学与免疫学专业委员会副主任委员。陈则研究员长期致力于流感病毒及其疫苗研究，曾担任国家“防治非典型肺炎专家组”和“防控高致病性禽流感专家组”成员，曾先后主持国家科技攻关项目、欧盟第七框架课题、“973”子课题、国家自然科学基金等课题三十余项。现主持国家“863”重点项目、国家“重大新药创制”科技重大专项、国家“重大传染病防治”科技重大专项等课题，并多次担任国家自然基金委、国家科技部项目和新药评审专家。近年来，在国际SCI源杂志上共发表相关论文80篇，并多次担任国际杂志审稿人。2009年，作为上海所甲流疫苗研发工作小组常务组长积极有效推动了甲流疫苗研发工作，对疫苗上市做了重要贡献，目前也承担了H7N9流感疫苗研发任务。
荣誉
2003年湖北省抗“非典”先进个人；
2004年武汉市优秀论文二等奖；
2006年湖南省优秀论文二等奖；
2007年湖南省科技进步一等奖；
2008年中国生物技术集团公司科技进步奖；
2010年上海市医务职工科技创新能手;
2012年上海市‘讲理想、比贡献’活动科技标兵”称号
学术报告
多次受邀在英国、德国、法国、韩国以及国内会议上作学术报告：
1，2012年分别受邀至日内瓦和南非参加世界卫生组织组织的流感病毒相关会议；
2，2010年2月，应邀参加世界卫生组织在日内瓦举办的甲流防控会议，并代表中国疫苗生产企业作相关报告；
3，2010年1月,作为大陆特派代表参加两岸共同防治H1N1新型流感研讨会并作题为甲型H1N流感病毒疫苗研究的报告。
论文
2005年到上海所任现职以来国际杂志发表论文目录（60篇）：（标*为通讯作者）
ChenQ,HuangS,ChenJ,ZhangS,ChenZ*.NAproteinsofinfluenzaAvirusesH1N1/2009,H5N1,andH9N2showdifferentialeffectsoninfectioninitiation,virusrelease,andcell-cellfusion.PLoSOne.2013;8(1):e54334.doi:10.1371/journal.pone.0054334.
WangG,ZhouF,BuchyP,ZuoT,HuH,LiuJ,SongY,DingH,TsaiC,ChenZ,ZhangL,DeubelV,ZhouP.DNAPrimeandVirus-likeParticleBoostfromaSingleH5N1StrainElicitsBroadlyNeutralizingAntibodyResponsesagainstHeadRegionofH5Hemagglutinin.JInfectDis.2013Aug2.[Epubaheadofprint]
WangB,ZhangH,ChenQ,ChenZ*.FullGenomeSequenceofanAvianInfluenzaH5N1VirusIsolatedfromtheEnvironmentinHunanProvince,China.GenomeAnnounc.2013May16;1(3).
ZhangF,FangF,ChangH,PengB,WuJ,ChenJ,WangH,ChenZ*.ComparisonofprotectionagainstH5N1influenzavirusinmouseoffspringprovidedbymaternalvaccinationwithHADNAandinactivatedvaccine.ArchVirol.2013Jun;158(6):1253-65
WangW,CaiJ,WuY,HuL,ChenZ,HuJ,ChenZ,LiW,GuoM,HuangZ.NovelactivityofKRABdomainthatfunctionstoreinforcenuclearlocalizationofKRAB-containingzincfingerproteinsbyinteractingwithKAP1.CellMolLifeSci.2013May12.[Epubaheadofprint]
ZhengD,YiY,ChenZ*.Developmentoflive-attenuatedinfluenzavaccinesagainstoutbreaksofH5N1influenza.Viruses.2012Dec10;4(12):3589-605.Review.
LuoJ,ZhengD,ZhangW,FangF,WangH,SunY,DingY,XuC,ChenQ,ZhangH,HuangD,SunB,ChenZ*.Inductionofcross-protectionagainstinfluenzaAvirusbyDNAprime-intranasalproteinbooststrategybasedonnucleoprotein.VirolJ.2012Nov23;9:286.doi:10.1186/1743-422X-9-286.
HuangS,ChenJ,ChenQ,WangH,YaoY,ChenJ,ChenZ*.AsecondCRM1-dependentnuclearexportsignalintheinfluenzaAvirusNS2proteincontributestothenuclearexportofviralribonucleoproteins.JVirol.2013Jan;87(2):767-78.doi:10.1128/JVI.06519-11.
ZhongdongYang,ShileiWang,WeiLi,ChangguiLi,JinrongDong,FangjunLi,ShuqiaoWang,WenqingChai,BingSun,ZeChen*.Thelong-termimmunogenicityofaninactivatedsplit-virion2009pandemicinfluenzaAH1N1vaccine:Randomized,observer-masked,single-centerclinicalstudy.ResultsinImmunology.Availableonline9October2012.
WangX,ZhangW,LiuF,ZhengM,ZhengD,ZhangT,YiY,DingY,LuoJ,DaiC,WangH,SunB,ChenZ*.Intranasalimmunizationwithliveattenuatedinfluenzavaccinepluschitosanasanadjuvantprotectsmiceagainsthomologousandheterologousviruschallenge.ArchVirol.2012;157(8):1451-61.
YaoYanfeng,WangHuadong,ChenQuanjiao,ZhangHongbo,ZhangTao,ChenJianjun,XuBing,WangHanzhong,SunBing,ChenZ*.CharacterizationoflowpathogenicH6N6avianinfluenzavirusesincentralChina.ArchVirol.2012Oct9.[Epubaheadofprint].
WangBa,ChenQuanjiaoandChenZ*.CompleteGenomeSequenceofaH9N2AvianInfluenzaVirusIsolatedfromegretinLakeDongtingWetland.JVirol.2012Nov;86(21):11939.
TaoL,ChenJ,ZhengZ,MengJ,ZhangZ,ChenY,LuoH,LiH,ChenZ,HuQ,WangH.H5N1influenzavirus-likeparticlesproducedbytransientexpressioninmammaliancellsinducehumoralandcellularimmuneresponsesinmice.CanJMicrobiol.2012Mar19.[Epubaheadofprint
XuC,HuWB,XuK,HeYX,WangTY,ChenZ,LiTX,LiuJH,BuchyP,SunB.Aminoacids473Vand598PofPB1fromanavianorigininfluenzaAviruscontributetopolymeraseactivityespeciallyinmammaliancells.JGenVirol.2012Mar;93(Pt3):531-40.Epub2011Nov16.
ZhangH,ChenQ,ChenZ*.CharacterizationofanH4N2avianinfluenzavirusisolatedfromdomesticduckinDongtingLakewetlandin2009.VirusGenes.2012Feb;44(1):24-31.Epub2011Aug19.
CuiZQ,RenQ,WeiHP,ChenZ,DengJY,ZhangZP,ZhangXE.Quantumdot-aptamernanoprobesforrecognizingandlabelinginfluenzaAvirusparticles.Nanoscale.2011Apr21.[Epubaheadofprint]
ZhangH,XuB,ChenQ,ChenJ,ChenZ*.CharacterizationofanH10N8influenzavirusisolatedfromDongtinglakewetland.VirolJ.2011Jan27;8(1):42.
XuK,LingZY,SunL,XuY,BianC,HeY,LuW,ChenZ,SunB.BroadHumoralandCellularImmunityElicitedbyaBivalentDNAVaccineEncodingHAandNPGenesfromanH5N1Virus.ViralImmunol.2011Feb;24(1):45-56
WeiJ,YanB,ChenZ,LiT,DengF,WangH,HuZ.ProductionandcharacterizationofmonoclonalantibodiesagainstthehemagglutininofH5N1andantigenicinvestigationofavianinfluenzaH5N1virusesisolatedfromChina.CanJMicrobiol.2011Jan;57(1):42-8
ZhangHongbo,XuBing,ChenQuanjiao,ChenZe*.CharacterizationofH9N2influenzavirusesisolatedfromDongtingLakewetlandin2007.ArchVirol.2011Jan;156(1):95-105.
WuJ,wangF,FangF,ZhangW,ChangH,ZhengLandChenZ*.SuperiorprotectionprovidedbyasingledoseofMF59-adjuvantedwholeinactivatedH5N1influenzavaccineintype1diabeticmice.ArchVirol.2011Mar;156(3):387-95.
XuK,KlenkC,LiuB,KeinerB,ChengJ,ZhengBJ,LiL,HanQ,WangC,LiT,ChenZ,ShuY,LiuJ,KlenkHD,SunB.ModificationofNonstructuralProtein1ofInfluenzaAVirusbySUMO1.JVirol.2011Jan;85(2):1086-98.
SuiZ,ChenQ,FangF,ZhengM,ChenZ*.Cross-protectionagainstinfluenzavirusinfectionbyintranasaladministrationofM1-basedvaccinewithchitosanasanadjuvant.Vaccine.2010Nov10;28(48):7690-8.
HeY,XuK,KeinerB,ZhouJ,CzudaiV,LiT,ChenZ,LiuJ,KlenkHD,ShuYL,SunB.InfluenzaAvirusreplicationInducesCellCycleArrestinG0/G1Phase.JVirol.2010Dec;84(24):12832-40.
ChangH,HuangC,WuJ,FangF,ZhangW,WangF,ChenZ*.AsingledoseofDNAvaccinebasedonconservedH5N1subtypeproteinsprovidesprotectionagainstlethalH5N1challengeinmicepre-exposedtoH1N1influenzavirusVirolJ.2010Aug21;7:197
GuoLina,ZhengMei,DingYahong,LiDongmei,YangZhongdong,WangHaiming,ChenQuanjiao,SuiZhiwei,FangFangandChenZe*.ProtectionagainstmultipleinfluenzaAsubtypesbyintranasaladministrationofrecombinantnucleoprotein.ArchVirol.2010Nov;155(11):1765-75.
ChenJ,HuangS,ChenZ*.HumancellularproteinnucleoporinhNup98interactswithinfluenzaAvirusNS2/NEPproteinandover-expressionofitsGLFGrepeatdomaincaninhibitviruspropagation.JGenVirol.2010Oct;91(Pt10):2474-84.
ChangH,LiX,TengY,LiangY,PengB,FangF,ChenZ*.ComparisonofAdjuvantEfficacyofChitosanandAluminumHydroxideforIntraperitoneallyAdministeredInactivatedInfluenzaH5N1Vaccine.DNACellBiol.2010Sep;29(9):563-8.
ZeChen*,ZhongdongYang.PandemicInfluenzaVaccineClinicalTrialAbstractMinimumInformation:SummaryonH1N1vaccineinChina:statemanufacturers.publishedontheWorldHealthOrganization(WHO)website(http://www.who.int/entity/vaccine_research/diseases/influenza/18_feb_ZeChen.pdf)2010，
LuB,HuangY,HuangL,LiB,ZhengZ,ChenZ,ChenJ,HuQ,WangH.Effectofmucosalandsystemicimmunizationwithvirus-likeparticlesofsevereacuterespiratorysyndromecoronavirusinmice.Immunology.2010Jun;130(2):254-61.
WuJ,ZhangF,FangF,ChangH,WangF,YangZ,SunB,ChenZe*.EfficacyofinactivatedvaccineagainstH5N1influenzavirusinfectioninmicewithtype1diabetes.Vaccine.2010Mar24;28(15)：2775-81
SuiZ,ChenQ,WuR,ZhangH,ZhengM,WangH,ChenZe*.Cross-protectionagainstinfluenzavirusinfectionbyintranasaladministrationofM2-basedvaccinewithchitosanasanadjuvant.ArchVirol.ArchVirol.2010Apr;155(4):535-44
LiangXF,WangHQ,WangJZ,FangHH,WuJ,ZhuFC,LiRC,XiaSL,ZhaoYL,LiFJ,YanSH,YinWD,AnK,FengDJ,CuiXL,QiFC,JuCJ,ZhangYH,GuoZJ,ChenPY,ChenZ,YanKM,WangY.Safetyandimmunogenicityof2009pandemicinfluenzaAH1N1vaccinesinChina:amulticentre,double-blind,randomised,placebo-controlledtrial.Lancet.2010Jan2;375(9708):56-66.
WuR,GuanY,YangZ,ChenJ,WangH,ChenQ,SuiZ,FangF,ChenZ*.AlivebivalentinfluenzavaccinebasedonaH9N2virusstrain.Vaccine.2010Jan8;28(3):673-80.
ChenJ,FangF,YangZ,LiuX,ZhangH,ZhangZ,ZhangX,ChenZ*.CharacterizationofhighlypathogenicH5N1avianinfluenzavirusesisolatedfrompoultrymarketsincentralChina.VirusRes.2009Dec;146(1-2):19-28.
HuangShengping,ChenJingjing,WangHuadong,SunBing,WangHanzhong,ZhangZhiping,ZhangXianen,ChenZ*.InfluenzaAvirusmatrixprotein1interactswithhTFIIIC102-s,ashortisoformofthepolypeptide3subunitofhumangeneraltranscriptionfactorIIIC.ArchVirol.2009;154(7):1101-10.
WuRui,ChenQuanjiao,ZhengLiyun,ChenJianjun,SuiZhiwei,ChenZe*.GenerationandevaluationofanH9N1influenzavaccinederivedbyreversegeneticsthatallowsutilizationofaDIVAstrategyforcontrolofH9N2avianinfluenza.ArchVirol2009;154(8):1203-10.
ZhengL,WangF,YangZ,ChenJ,ChangH,ChenZ*.AsingleimmunizationwithHADNAvaccinebyelectroporationinducesearlyprotectionagainstH5N1avianinfluenzaviruschallengeinmice.BMCInfectDis.2009Feb12;9:17.
ChenJ,YangZ,ChenQ,LiuX,FangF,ChangH,LiD,ChenZe*.CharacterizationofH5N1influenzaAvirusesisolatedfromdomesticgreen-wingedteal.VirusGenes.2009Feb;38(1):66-73.
ChenQ，KuangH,WangH,FangF,YangZD,ZhangZh,ZhangX,ChenZ*.Comparingtheabilityofaseriesofviralprotein-expressingplasmidDNAstoprotectagainstH5N1influenzavirus.VirusGenes.2009,38:30–38.
LuB,TaoL,WangT,ZhengZ,LiB,ChenZ,HuangY,HuQ,WangH.Humoralandcellularimmuneresponsesinducedby3aDNAvaccinesagainstsevereacuterespiratorysyndrome(SARS)orSARS-likecoronavirusinmice.ClinVaccineImmunol.2009Jan;16(1):73-7.
ZhouY,FangF,ChenJ,WangH,ChangH,YangZ,ChenZe*.ElectroporationatLowVoltagesEnablesDNAVaccinetoProvideProtectionagainstaLethalH5N1AvianInfluenzaVirusChallengeinMice.Intervirology.2008Sep24;51(4):241-246.
WangFuyan,YangWen,FangFang,ChangHaiyan,YuPing,ChenZe*.EssentialsequenceofinfluenzaBvirushemagglutininDNAtoprovideprotectionagainstlethalhomologousviralinfection.DNAandcellbiology2008Jul;27(7):377-85.
WangW,CuiZQ,HanH,ZhangZP,WeiHP,ZhouYF,ChenZ.ZhangXE.ImagingandcharacterizinginfluenzaAvirusmRNAtransportinlivingcells.NucleicAcidsRes.2008Sep;36(15):4913-28.
FangF.,CaiXQ,ChangHY.,WangHD,YangZD,ChenZ*.ProtectionabilityofinfluenzaBvirusDNAVaccinesexpressinghamagglutinin,neuraminidaseorbothinmice.Actavirologica52:107–112,2008
WuR,SuiZW,ZhangHB,ChenQJ,LiangWW,YangKL,XiongZL,LiuZW,ChenZ*,XuDP.CharacterizationofapathogenicH9N2influenzaAvirusisolatedfromcentralChinain2007,ArchVirol.2008;153(8):1549-55
BaiB,LuX,MengJ,HuQ,MaoP,LuB,ChenZ,YuanZ,WangH.VaccinationofmicewithrecombinantbaculovirusexpressingspikeornucleocapsidproteinofSARS-likecoronavirusgenerateshumoralandcellularimmuneresponses.MolImmunol.2008Feb;45(4):868-75.
ChenJ,ZhangF,FangF,ChangH,ChenZ*.VaccinationwithhemagglutininorneuraminidaseDNAprotectsBALB/cmiceagainstinfluenzavirusinfectioninpresenceofmaternalantibody.BMCInfectDis.2007Oct16;7(1):118.
ChenZ*,WatanabeS.Anintrastrainvariationofa189-bprepeatregioninthehumancytomegalovirusreplicationorigin.ActaVirol.2007;51(1):21-6.
LuX,ChenY,BaiB,HuH,TaoL,YangJ,ChenJ,ChenZ,HuZ,WangH.Immuneresponsesagainstsevereacuterespiratorysyndromecoronavirusinducedbyvirus-likeparticlesinmice.Immunology.2007Dec;122(4):496-502.
HuH,LuX,TaoL,BaiB,ZhangZ,ChenY,ZhengF,ChenJ,ChenZ,WangH.InductionofspecificimmuneresponsesbySARS-CoVspikeDNAvaccinewithorwithoutinterleukin-2immunizationusingdifferentimmuneroutesinmice.ClinVaccineImmunol.2007Jul;14(7):894-901.
YuY,JinH,ChenZ,YuQL,MaYJ,SunXL,WangB.Children'svaccinesdonotinducecrossreactivityagainstSARS-CoV.JClinPathol.2007Feb;60(2):208-11.
QiuM,FangF,ChenY,WangH,ChenQ,ChangH,WangF,WangH,ZhangR,ChenZ*，ProtectionagainstavianinfluenzaH9N2viruschallengebyimmunizationwithhemagglutinin-orneuraminidase-expressingDNAinBALB/cmice.BiochemBiophysResCommun.2006May19;343(4):1124-31.
LiX,FangF,SongY,YanH,ChangH,SunS,ChenZ*，EssentialsequenceofinfluenzaneuraminidaseDNAtoprovideprotectionagainstlethalviralinfection.DNACellBiol.2006Apr;25(4):197-205.
FenghuaZhang,JianjunChen,FangFang,YuZhou,JianWu,HaiyanChang,RanZhang,FuyanWang,XiaomanLi,HanzhongWang,GuohuaMaandZeChen*，Maternalimmunizationwithbothhemagglutinin-andneuraminidase-expressingDNAsprovideanenhancedprotectionagainstalethalinfluenzaviruschallengeininfantandadultmice.DNACellBiology.2005;24(11):758-765.
GuoY,SunS,WangK,ZhangS,ZhuW,ChenZ.ElicitationofimmunityinmiceafterimmunizationwiththeS2subunitofthesevereacuterespiratorysyndromecoronavirus.DNACellBiol.2005Aug;24(8):510-5.
JianjunChen,FangFang,XiangzhongLi,HaiyanChangandChenZe*.ProtectionagainstinfluenzavirusinfectioninBALB/cmiceimmunizedwithasingledoseofneuraminidase-expressingDNAsbyelectroporation.Vaccine.2005;23(34):4322-4328.
HuW,BaiB,HuZ,ChenZ,AnX,TangL,YangJ,WangH,WangH.Developmentandevaluationofamultitargetreal-timeTaqmanreversetranscription-PCRassayfordetectionofthesevereacuterespiratorysyndrome-associatedcoronavirusandsurveillanceforanapparentlyrelatedcoronavirusfoundinmaskedpalmcivets.JClinMicrobiol.2005May;43(5):2041-6.
ZhuQ,ChangH,ChenY,FangF,XueC,ZhangF,QiuM,WangH,WangB,ChenZe*.Protectionofinactivatedinfluenzavirusvaccineagainstlethalinfluenzavirusinfectionindiabeticmice.BiochemBiophysResCommun.2005Apr1;329(1):87-94.
JinH,XiaoC,ChenZ,KangY,MaY,ZhuK,XieQ,TuY,YuY,WangB.InductionofTh1typeresponsebyDNAvaccinationswithN,M,andEgenesagainstSARS-CoVinmice.BiochemBiophysResCommun.2005Mar25;328(4):979-86.
专著
《分子病毒学》，流行性感冒病毒章节，黄文林主编，人民卫生出版，2002年，2005年再版
《生物化学与分子生物学实验教程》，第四章节，约2万字，梁宋平主编，高等教育出版社，2003年
《疫苗研究与应用》，流感病毒疫苗章节，约4万字,赵铠主编，人民卫生出版社，2013年