杨爱芳 教授
博士生导师
Tel:(86) 531-88364350
E-mail:nihaohua@263.net
教育背景
Aifang Yang
Professor of Cell Biology and Genetics
School of Life Science (86) 531-88364350(phone)
Shandong University (86) 531-88564350 (FAX)
Jinan, 250100, P. R. China nihaohua@263.net (E-mail)
EDUCATION
Ph.D. Shandong University, December 2003 (Developmental Biology)
Dissertation title: “Breeding salt-tolerant materials with elite agronomic traits for Beta vulgaris L. via bioengineering techniques”.
M.S. Shandong University, September 1996 (Cell Biology)
Thesis title: “Changes of Ca2+ and soluble proteins during the dedifferentiation of cultured cells of Linum usitatissimum L.”.
B.S. Shandong University, September 1993 (Botany)
Thesis title: “The changes of phenol complexes during the culture of cotton calli”.
EMPLOYMENT
2007.11-2008.12 Visiting Research Scholar, Plant Biology & Pathology Department, Rutgers University （State University of New Jersey）
2006-current, Professor, Institute of Plant Cell & Genetics, School of Life Science, Shandong University
2001-2006, Associate Professor, Institute of Plant Cell & Genetics, School of Life Science, Shandong University
1998-2001, Instructor, Department of Biochemistry, School of Life Science, Shandong University
1996-1998, Teaching Assistant, Department of Biochemistry, School of Life Science, Shandong University
RESEARCH EMPHASIS
Gene Cloning and Molecular Breeding
In the past years, I had been engaged in plant biotechnology researches and accumulated plenty experiences in tissue culture, plantlet regeneration, somaclonal variant selection, gene identification and genetic transformation of crops including maize, beet, wheat and cotton; and Gramineae foliages of perennial ryegrass (Lolium perenne L.), annual ryegrass (Lolium multiflorum Lam.), common orchardgrass (Dactylis glomerata L.), tall fescue (Festuca arundinacea Schreb.), Kentucky bluegrass (Poa pratensis L.), Lawngrass (Zoysia japonica Steud) and so on. We had established and optimized Agrobacterium-mediated transformation protocols using either calli or multiple buds or shoots in vitro or even the naked meristem of shoot tips in vivo, bypassing the in vitro culture process, as recipients, and genes involved in abiotic stress resistance, sugar or starch metabolic pathways or development and nutrition utilization and so on such as betA, AtNHX1,DREB1A, DREB2A, PIS, PPase,1-fft,1-sst, galE, lgtC, etc, cloned mainly from salt cress, maize, lettuce and yeasts, respectively, were transferred into these materials and transgenic plants and progenies with modified characters were obtained, respectively, to meet the agricultural or horticultural need. Moreover, the transgene expression profile, heredity stability in the progenies and the effects of the transgene expression on the transgenic plants characters were also one of my majors.
Plant Biological Reactor
Compared to other biological reactors, like microbes and animal cell expression systems, plants have unique advantages. Therefore, transgenic plants as a new biological reactor posses great potentials. Using plant to produce biologically active products, including valuable proteins, carbohydrates for clinical, food and industrial purposes is the goal of our research. Modification of the carbohydrate metabolic pathways in plants using genetic approaches might transform plants into biological reactors to produce valuable oligo-, poly-saccharide and glycoconjugates. Gene involved in carbohydrate metabolic pathways and in starch synthesis pathways have been identified and transferred into tobacco，beet and maize, respectively, and inspiring results have been obtained.
Grants
1. AF Yang, JR Zhang, KP Li et al. Creation of new maize germplasm with efficient phosphorus-utilization traits via genetic manipulation (2008AAZX08003-005), funded by Ministry of Agriculture of the People’s Republic of China, ￥4,200,000, 2008-2010.
2. AF Yang, JR Zhang, KW Zhang, XF Gu et al. Creation of new germplasm for breeding of supermaize (2007BAD31B01), funded by Ministry of Science & Technology of the People’s Republic of China, ￥6,000,000, 2007-2010.
3. AF Yang, JR Zhang, XF Gu et al. Screening and planting of sea-water resistant varieties in the beach of yellow river delta (2007AA091701), High Tech-Research and Development Program (863) of China, funded by Ministry of Science & Technology of the People’s Republic of China, ￥700,000, 2007-2010.
4. Juren Zhang, Aifang Yang, Kewei Zhang et al. Functional genomics of stress-, desease-resistance and seed quality in maize (2007AA10A107). High Tech-Research and Development Program (863) of China, funded by Ministry of Science & Technology of the People’s Republic of China, ￥ 研究成果及发表论文
Publications
2008:
1. CR Wang, AF Yang, GD Yue, Q Gao, HY Yin, JR Zhang. Enhanced expression of phospholipase C1 (ZmPLC1) improves drought tolerance in transgenic maize. Planta, 2008, 227: 1127-1140. SCI.IF 3.06
2. CR Wang, AF Yang, HY Yin, JR Zhang. Influence of Water Stress on Endogenous Hormone Contents and Cell Damage of Maize Seedlings. Journal of Integrative Plant Biology, 2008, 50 (4): 427-434. SCI.IF 0.67
3. LZ Wang, AF Yang, CM He, ML Qu, JR Zhang. Creation of new maize germplasm using alien introgression from Zea mays ssp. exicana. Euphytica, 2008, 164: 789-801
4. KP Li, CZ Xu, ZX Li, KW Zhang, AF Yang, JR Zhang. Comparative proteome analyses of phosphorus responses in maize (Zea mays L.) roots of wild-type and low-P tolerance mutant reveal root characteristics associated with P-efficiency. The Plant Journal, 2008, 55：927-939. SCI, IF 6.75
5. ZH Sui, LY Niu, GD Yue, AF Yang, JR Zhang, Cloning and expression analysis of some genes involved in the phosphoinositide and phospholipid signaling pathways from maize (Zea mays L.). Gene, 2008, doi:10.1016/j.gene.2008.09.004. SCI, IF.2.87
2007:
6. KP Li, CZ Xu, KW Zhang, AF Yang, JR Zhang*. Proteomic analysis of roots growth and metabolic changes under phosphorus defecit in maize (Zea mays L.) plants. Proteomics, 2007, 2007, 7(9): 1501-1512.
7. SL Lv, AF Yang, KW Zhang, L Wang, JR Zhang*. Increase of glycinebetaine synthesis improves drought tolerance in cotton. Molecular Breeding, 2007, 20: 233-248.
8. XG Duan, AF Yang, F Gao, SLZhang, JR Zhang*. Heterologous expressing vacuolar H+-PPase enhanced electrochemical gradient across vacuolar membrane and improved tobacco cell salt tolerance. Protoplasm, 2007, 232(1-2): 87-95
9. KP Li, ZP Xug, KW Zhang, AF Yang, JR Zhang. Efficient production and characrerization for maize inbred lines with low-phosphorus tolerance. Plant Science, 2007, 172: 255-264.
10. AF Yang, KW Zhang, XY Yin, JR Zhang*. Analysis of agronomic traits and combining ability of transgenic plants in maize. Scientia Agricultura Sinica, 2007, 40 (12): 2895-2902 (in Chinese).
11. HJ Li, AF Yang, XC Zhang, F Gao, JR Zhang. Improving freezing tolerance of transgenic tobacco expressing sucrose: sucrose 1-fructosyltransferase gene from Lactuca sativa. Plant Cell, Tissue, Organ Culture, 2007, 2007, 89: 37-48.
2006:
12. F Gao, Q Gao, XG Duan, GD Yue, AF Yang*, JR Zhang. Cloning of an H+-PPase gene from Thellungiella halophila and its heterologous expression to improve salt-tolerance of tobacco. Journal of Experimental Botany, 2006, 57(12): 3256-3270.
13. AF Yang, CM He, KW Zhang, JR Zhang*. Improvement of Agrobacterium-mediated transformation of embryogenic calli from maize elite inbred. In Vitro Cellular and Development Biology - Plant, 2006, 42(3): 215-219.
14. AF Yang, SL Zhao, XY Yin, F Gao, JR Zhang. Introduction of AtNHX1 into beet improved salt-tolerance of transgenic plants. High Technology Letters, 2006, 12(2): 182-187.
2005:
15. AF Yang, XG Duan, XF Gu, F Gao, JR Zhang*. Efficient transformation of beet (Beta vulgaris L.) and production of plants with improved salt-tolerance. Plant Cell, Tissue, Organ Culture, 2005, 83: 259-270.
16. XR Hu, AF Yang, KW Zhang, J Wang, JR Zhhang*. Optimization of in Vitro Multiple Shoot Clump Induction and Plantlet Regeneration of Kentucky bluegrass (Poa pratensis L.). Plant Cell, Tissue, Organ Culture, 2005, 83: 342-350.
17. XF Gu, AF Yang, H Meng, JR Zhang. In vitro induction of tetraploid plants from diploid zhanhua winter jujube (Zizyphus Jujuba Mill.). Plant Cell Reports, 2005, 24: 671-676.
18. YS Zhang, SM Zhai, AF Yang, JR Zhang. Maize transformation via pollen tube pathway and the inheritance of transgene in progeny. High Technology Letters, 2005, 11(2): 202-206. (in Chinese).
19. SM Zhai, ZH Sui, AF Yang, JR Zhang. Characterization of a novel phosphoinositide-specific phospholipase C from Zea mays and its expression in Escherichia coli. Biotechnology Letters, 2005, 27: 799-804.
20. YS Zhang, XY Yin, AF Yang, GS Li, JR Zhang*. Stability of inheritance of transgenes in maize (Zea mays L.) lines produced using different transformation methods. Euphytica, 2005, 144: 11-22.
21. F Gao, Q Gao, GD Yue, AF Yang, JR Zhang*. Cloning of CBF1 gene from Thellungiella salsuginea. Journal of Shandong University, 2005, 40: 113-120. (in Chinese).
22. H Zhang, XG Duan, AF Yang*, JR Zhang. Optimization of transformation conditions in shoot clumps of Beta maritima L. mediated by Agrobacterium tumefaciens. Sugar Crops of China, 2005, 1: 1-5. (in Chinese).
23. H Zhang, XG Duan, AF Yang*, JR Zhang. Genetic transformation of Beta maritima L. by Agrobacterium tumefaciens and production of salt tolerant transgenic plants. High Technology Letters, 2005, 15(8): 72-77. (in Chinese).
24. AF Yang, SY Jiang, XR Hu, XG Duan, JR Zhang*. Efficient in vitro propagation and salt tolerant plantlet regeneration in Rumex. Acta Prataculturae Sinica, 2005, 14(2): 43-47. (in Chinese)
25. SY Jiang, XR Hu, XG Chao, AF Yang*. Efficient production of multiple shoots and inflorescences induction from shoot of lolium multiflorum L. and lolium perenne L. Acta Prataculturae Sinica, 2005, 14(6): 100-106. (in Chinese)
26. XR Chao, LL Song, XRi Hu, SY Jiang, AF Yang*. High efficiency induction of multiple shoots from orchardgrass (Dactylis glomerata L.) and regeneration of salt-tolerant plantlets. Shandong Agricultural Science, 2005, 1: 7-10. (in Chinese)
2004:
27. AF Yang et al. Induction of multiple bud clumps from inflorescence tips and regeneration of salt-tolerant plantlets in Beta vulgaris L. Plant Cell, Tissue, Organ Culture, 2004, 77: 29-34.
28. XY Yin, AF Yang, KW Zhang, JR Zhang. Production and analysis of transgenic maize with improved salt-tolerance by introduction of AtNHX1 gene. Acta Botanica Sinica, 2004, 46(7): 854-861.
29. SY Jiang, AF Yang, XR, JR Zhang. Tissue culture and plantlet regeneration of Rumex. Plant Physiology Communication, 2004, 40(1): 61. (in Chinese)
30. AF Yang, CM He, XL Wang, JR Zhang. In vitro culture and plant regeneration from immature inflorescences of perennial ryegrass（Lolium perenne L.）and Italian ryegrass（Lolium multiflorum Lam.）varieties. Acta Prataculturae Sinica, 2004, 13(5): 84-90. (in Chinese)
31. CM He, XL Wang, SY Jiang, XR Hu, AF Yang. Induction of calli from young ears and immature embryos of Rye Dongmu 70 and regeneration of plantlets. Acta Prataculturae Sinica, 2004, 13(6): (in Chinese)
32. H Zhang, AF Yang, XG Duan, JR Zhang. High efficient induction of multiple bud clumps and regeneration of plantlets for Beta maritima L. Sugar Crops of China, 2004, 1: 1-4. (in Chinese)
2003:
33. AF Yang, LP Zhu, XG Duan, F gao, JR Zhang. Transformation of sugar beet with betA gene improved salt-tolerance of transgenic plants. Collection of thesis of high-tech forum of marine organisms, 2003, 923-933. (in Chinese)
34. XY Wang, XY Shan, AF Yang, JR Zhang. Construction of plant expression vector and analysis of herbicide-resistance and salt-tolerance of transgenic tobacco. Journal of Agricultural Biotechnology. 2003, 11(6): 554-560. (in Chinese)
2002:
35. AF Yang, SL Zhao, LP Zhu, SM Zhai, JR Zhang, Yue Huang, Xiaoling Zhai. The creation of new sugar beet germplasm tolerant to salt by genetic transformation. Shandong Agricultural Science, 2002 (2): 3-6. (in Chinese)
2001:
36. LP Zhu, AF Yang, JR Zhang, KS Chen. Multiple shoot induction and plantlet regeneration in sugar beet. High Technology Letters, 2001, 11(125): 17-20. (in Chinese)
37. GS Li, AF Yang, JR Zhang, YP Bi, L Shan. Genetic transformation of calli from maize and regeneration of herbicide-resistant plantlets. Chinese Science Bulletin, 2000, 45：2181-2184 (in Chinese); 2001, 46(7): 563-566 (in English).
2000:
38. XH Li, DL Li,JRn Zhang, AF Yang. Somatic embryogenesis and plant regeneration in high frequencies from cultured shoot apices of Zea mays. Plant Physiology Communications, 2000, 36(5): 430-433. (in Chinese)
39. TG Li, AF Yang, KW Zhang, JR Zhang. Selection of mutants resistant to Fusarium graminearum and studies on the progenies of the regenerated plants. Scientia Agricultura Sinica, 2000, 33(supplement): 147-151. (in Chinese)
1999:
40. XF Wang, AF Yang, JR Zhang. Genetic transformation of protoplasts from Peucedanum terebinthaceum and regeneration of berbicide-resistant plantlets. Acta Botanica Sinica, 1999, 41(7): 706-710. (in Chinese)
1998:
41. JR Zhang, SF Gao, AF Yang, JJ Yu. Selection of resistant mutants for Helminthosporium maydis via tissue culture in maize. Chinese Journal of Biotechnology, 1998, 14(4): 456-459. (in Chinese)
Patents:
1. AF Yang, JR Zhang, KW Zhang, HF Wang. A method for creating new salt-toleant germplasm through variation and selection of beet multiple buds. Chinese patent, ZL02135731.5, 2004.
2. AF Yang, JR Zhang, XY Yin, KW Zhang, XF Gu. A method and its utilization for establishing a recipient system for genetic transformation of ryegrass. Chinese patent, ZL 200410024550.3, 2006.
3. JR Zhang, AF Yang, KW Zhang, XY Yin, XF Gu. A method and its utilization for establishing a genetic transforming system for Kentuky bluegrass. Chinese patent, ZL200410024537.8, 2007.
4. JR Zhang, M Shang, XH Li, AF Yang, QW Zhangi. A method and its utilazation for transforming plants with macroseeds. Chinese patent, ZL01104428.4, 2005.
5. JR Zhang, AF Yang, XF Gu, KW Zhang, XY Yin. A method for improving salt- and drought-tolerance of maize and wheat via pyramiding betA、NHX1、Ppase gene. Chinese patent, ZL 200510044791.9, 2007.